Product Name: Alpha-Co-Culture Medium, EF, Kit
Catalog Number: PG010
Product Format: Liquid
Storage: 2-8° C.
General Information: Alpha-Co-Culture Medium, EF, Kit
Alpha-Co-culture medium to culture human fibroblasts with/and HUVECs
Alpha-Co-Culture Medium Kit is a complete medium formulated for optimal growth
of co-cultures of fibroblasts isolated out of most organs and HUVECs. The medium
contains essential and non-essential amino acids, vitamins, organic and inorganic
compounds, hormones, growth factors, trace minerals plus 2% FCS together with
the supplement KIT.
The medium is for research use only and not approved for human or animal use or
for application in any in vitro diagnostic procedure.
Storage of the supplement:
Please note that the basal medium has to be stored at 4°C up to 8°C but never
should be frozen. The supplements have to be stored at -20°C. Protect from light for
a longer time period.
Preparing and Stability:
Thaw the supplement KIT at 37°C before adding to the basal medium. After thawing
please rinse the bottle and the tubes with 70% ethanol as protection. Remove
ethanol excess before opening the caps of the tubes. After adding the supplements
to the basal medium slew carefully to get a homogenous medium. Please be careful
not to touch the tube or bottle interior threads with your fingers. Please avoid long
term exposure to light because some components are light sensitive. Also do not
warm up the medium over 37°C. All this protects your medium against
contamination or degradation of essential components. Stored in the dark at 4°C
the reconstituted medium will be stable for 5 weeks.