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mouse brain tissue lysate - total protein C-LYSATE0012

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Mouse brain tissue is widely used in neuroscience research due to its structural and functional similarity to the human brain. It serves as a model for understanding neurological disorders, molecular pathways, and cellular mechanisms. Extracting key biomolecules from this tissue is made possible through the preparation of a mouse brain tissue lysate, a process that involves breaking down brain tissue to release its proteins, enzymes, and other molecular components. These lysates are essential for studies in proteomics, biomarker discovery, and drug development.

Mouse Brain Tissue Lysate Protocol

A proper mouse brain tissue lysate protocol ensures the efficient extraction of biomolecules while maintaining their integrity. The process typically includes the following steps:

  1. Tissue Collection: Fresh or frozen mouse brain tissue is obtained, ensuring minimal degradation.
  2. Homogenization: The tissue is homogenized using lysis buffer containing detergents, protease inhibitors, and phosphatase inhibitors to preserve proteins and enzymatic activity.
  3. Centrifugation: The homogenate is centrifuged to separate cellular debris from the supernatant, which contains the lysate.
  4. Storage: The lysate is stored at -80°C for long-term use in experiments.

Following an optimized protocol ensures consistent results across different research studies, providing reliable data for exploring brain-specific pathways.

Mouse Brain Cell Types

The mouse brain consists of various mouse brain cell types, each playing a unique role in its overall function. The main cell types include:

  • Neurons: Specialized for signal transmission through synapses.
  • Astrocytes: Supportive glial cells involved in maintaining the blood-brain barrier and providing nutrients to neurons.
  • Microglia: Immune cells of the brain responsible for clearing debris and responding to injury.
  • Oligodendrocytes: Produce myelin, which insulates neuronal axons for faster signal conduction.
  • Endothelial Cells: Form the blood vessels within the brain, regulating the exchange of substances between the bloodstream and brain tissue.

Each cell type contributes to the brain’s intricate functionality, making mouse models indispensable for studying these roles in health and disease.

How Many Cells Are in the Mouse Brain?

The mouse brain contains approximately 71 million cells, with about 40 million being neurons and the rest comprising various types of glial cells and other supporting cells. This cellular diversity is one reason why the mouse brain is an excellent model for exploring complex neurological processes, including development, aging, and disease progression.

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