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Product Description/Introduction:

RNase inactivation: We are thrilled to introduce our highly advanced RNase Inactivation reagent, which is expertly crafted to eliminate RNase activity irreversibly. This remarkable reagent guarantees that every piece of laboratory equipment and consumables is entirely free from RNase contamination following the inactivation procedure, making it an indispensable asset for various RNA-related applications. Whether RNA isolation, real-time PCR, Northern blotting, or fluorescence in situ hybridization (FISH), you can proceed with your experiments confidently, assured of accurate and reliable results.

Storage and Shipping Conditions:

Our robust RNase Inactivation reagent is conveniently shipped at room temperature to ensure optimal condition upon arrival. For maximum effectiveness, we recommend storing it at -20℃. With a generous shelf life of 12 months, you can rely on our RNase Inactivation reagent as a steadfast component in your molecular biology toolkit. Embrace the peace of mind and confidence of using this top-tier product” RNase removal.

Assay Protocol / Procedures for RNase Inactivation:

To ensure the effective inactivation of ribonuclease (RNase) and prevent potential contamination in your experimental setup, please follow the detailed steps below:

1.Preparation:

   - Gather all experimental consumables, including but not limited to pipette tips, RNA extraction tubes (e.g., EP tubes), and any other tools that will encounter RNA samples.

2.RNase Inactivation Treatment:

   - Fully immerse the collected consumables in the RNase Inactivation Reagent. Ensure that all surfaces of the items are adequately coated with the reagent to maximize effectiveness.

3. Incubation Process:

   - Place the immersed items in a suitable incubator set to a stable temperature of 60°C. It is essential to maintain this temperature throughout the incubation period.

Allow the items to be incubated for a minimum of 10 minutes. This duration is crucial for ensuring complete RNase inactivation and guaranteeing the integrity of subsequent RNA analyses.

Additional Notes:

1. The RNase Inactivation product is designed to be safe and non-corrosive, making it compatible with downstream procedures involving sensitive RNA analysis.

2. For efficiency, you can treat the same items multiple times within the same day if further inactivation is necessary, ensuring a continuous safeguard against RNase contamination.

3. Consistently verify that the incubation period is strictly adhered to, as any shorter duration may leave residual RNase activity, which could compromise the accuracy of your results.

4. Exercise caution when handling chemicals by minimizing direct contact. Always wear appropriate personal protective equipment (PPE), including safety glasses, disposable gloves, and protective clothing, to ensure safety and maintain a contamination-free environment during laboratory work.

"RNase Inactivating Solution" refers to a chemical solution used in molecular biology to inactivate ribonucleases (RNases), enzymes that degrade RNA. RNases are widely present and can pose a significant challenge during RNA-related experiments, as they can potentially destroy RNA samples, leading to inaccurate results. An RNase Inactivating Solution is specifically formulated to neutralize or eliminate RNase activity, ensuring RNA integrity during extraction, purification, and analysis.

Storage and Shipping Conditions